Effects of M2000 and Vitamin D3 in the Expression of IL-10, IFN-γ and IL-6 in Crohn’s Diseases
Keywords:
Vitamin D3, IL-6, IFN-γ, IL-10, M2000, Crohn’s diseaseAbstract
Background: Crohn’s disease is a chronic, transmural immune-mediated inflammatory disease of the gastrointestinal tract
(GIT). It can attack any portion of the GIT, although inflammation most commonly occurs in the lower portion of the small
intestine, known as the ileum. The disease can cause ulcerations within the intestine, which is capable of eroding into surrounding tissues, such as the bladder, vagina and the surface of the skin. Inflammation in Crohn’s disease is not limited to the
intestine, some Crohn’s disease patients have inflammation of the eyes and joints as well. This research aimed to assess the
efficacy of M2000 and vitamin D3 in the treatment of Crohn’s disease.
Materials and Methods: Fifteen (15) ml of blood was collected from 40 research participants. The PBMC was isolated, then
stimulated with LPS and incubated for 4 hours. The cells were later treated with various doses of M2000 and vitamin D3 and
incubated for 24 hours at optimum condition. The cells were harvested and centrifuged at 12,000g for 15 minutes. RNA was
extracted and converted to cDNA. Real-time PCR and ELISA were used for gene and cytokine expressions, respectively.
Results: The baseline expressions of IL-6 and IFN-γ were significantly upregulated in the positive controls in contrast to IL Conclusion: The result indicated that M2000 and vitamin D3 are potent immunosuppressive and immunomodulatory agents,
that may be used in Crohn’s disease management. It recommended that further researches and clinical tr
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