Serum-Free Expansion Media and Genomic Stability of Human Mesenchymal Stromal Cells: A Review
DOI:
https://doi.org/10.5281/zenodo.22237878Keywords:
mesenchymal stromal cells; serum-free medium; platelet lysate; genomic stability; senescenceAbstract
Background: Clinical doses of mesenchymal stromal cells require weeks of expansion, historically in fetal bovine serum.
Replacing it raised a second question: whether culture destabilises the genome.
Objective: To review human platelet lysate and defined serum-free media as replacements for fetal bovine serum, and the
evidence on genomic stability.
Methods: Narrative review of comparative culture studies and of cytogenetic, array and sequencing analyses of expanded
MSCs.
Findings: Platelet lysate matches or exceeds fetal bovine serum for proliferation and supports clinical-scale production, with
heparin and residual fibrinogen the technical constraints. The early reports of spontaneous malignant transformation
reflected cross-contamination with tumour lines, and one was retracted; aneuploidy occurs without transformation.
Conclusions: The ceiling on expansion is replicative senescence, not malignant transformation
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