Chondroinductive Activity from Commercially Available Demineralized Bone Matrix Sponge on Mesenchymal Stem Cells
Keywords:
Mesenchymal stem cell, Demineralized bone matrix, Chondro-inductive activityAbstract
osteoarthritis (OA) evolution occurs due to the limited regenerative capacity of articular chondrocytes, which leads to the loss
of function of the joint. For this reason our group working to develop new strategies to treat this disease. This study aimed
to demonstrated the feasibility of using a human-based scaffold for the induction of mesenchymal stem cell to chondrocytes.
The ATTC-PCS-500-12 (human mesenchymal stem cells, MSC) line was seeded in a human-scaffold commercially produced in
a tissue bank in the presentation of a demineralized bone matrix sponge (DBMs), they were cultivated for 14 days under two
in vitro conditions: i) culture medium with 10% of FBS, and ii) culture medium with 10% of FBS plus BMP-7 100 ng/ml. After
the period of cell culture, an immunofluorescence assay was carried out for observing the expression of the characteristic
proteins of the articular chondrocytes: SOX9, AGGRECAN and COL2A1. The results showed that the proteins expression was
similar for the two in vitro conditions tested. In conclusion we established that the use of DBMs scaffold encourages the MSC
differentiation to pheno
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