High-Fat Diet Reverses Neuroprotection Attained by Moderate Aerobic Exercise in the Spastic Han-Wistar Rat, a Model of Ataxia
Keywords:
cerebellum, BDNF, ataxia, treadmill, Purkinje cellsAbstract
Diet and moderate exercise may promote effective therapies for patients with neurodegenerative diseases; however, administering a high-fat diet may exacerbate disease symptoms. To examine the effect of high-fat diet on exercise-induced neuroprotection, a high-fat diet was provided to spastic Han-Wistar (sHW) rats (Rattus norvegicus), a model of ataxia. Previously,
we discovered that exercise led to improved motor activity and longevity, but not weight gain in the sHW mutant. What effect
would a high-fat diet be on exercising mutants? Mutant and normal male siblings at 30 days of age were given either a highfat diet (HF; 58% fat calories) or commercial rat chow diet (RC; 13% fat calories), and half of the rats were run on a treadmill
at 15m/min for 30 min at a 15o
incline for five days/week. While weight gain was similar among the exercising or sedentary
mutants regardless of diet, a significant increase in longevity of the mutants feeding on the RC compared to the HF-fed mutants
was observed. Normal rats were unaffected by consuming either diet. Brain-derived neurotrophic factor (BDNF) has been
reported to be elevated after acute exercise. Hence, we investigated whether there was a correlation between the effect of HF
treatment and BDNF expression quantified using an enzyme-linked immunosorbent assay (ELISA). Our results showed that
BDNF levels in the cortex, hippocampus and cerebellum were affected by both diet and exercise (p=0.009). The benefits of this
research could po
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