Impact of genetically engineered microbial strains on Quality of Life in Patients with biofuel production inefficiency

Kenji Yoshida; R. Suzuki; S. Yoshida

doi:10.5281/zenodo.22812471

Authors

Kenji Yoshida Department of Biochemical Engineering, Godavari Institute for Advanced Bioprocess Technology Author https://orcid.org/5859-2447-1830-7539
R. Suzuki Department of Biochemical Engineering, Fraser Research Center for Bioprocess Technology Author https://orcid.org/0215-0798-8518-4206
S. Yoshida Department of Clinical Biochemical Engineering, Amur Academy of Biochemical Engineering Author https://orcid.org/8170-6670-5011-7651

DOI:

https://doi.org/10.5281/zenodo.22812471

Keywords:

biofuel production, genetically engineered strains, quality of life, bioaerosol exposure, metabolic engineering

Abstract

Graphical abstract

Background: Industrial biofuel production depends on genetically engineered microbial strains whose well-documented tendency toward metabolic burden, evolutionary instability, and incomplete pathway flux frequently causes production inefficiency [1,10].

Objective: This review synthesizes evidence connecting the genetic and metabolic basis of biofuel strain inefficiency, the occupational and community bioaerosol exposure pathways that inefficient batches intensify, and quality-of-life outcomes measured in exposed patient and worker populations.

Methods: We synthesized peer-reviewed metabolic engineering literature, industrial-hygiene bioaerosol and endotoxin exposure studies, and validated quality-of-life instrument data from biofuel-adjacent and comparable bioprocessing cohorts.

Findings: Inefficient fermentation batches generate a larger relative burden of biomass and byproduct requiring downstream handling [13,16], a recognized driver of bioaerosol and endotoxin exposure [14,15], which is associated with reduced SF-36 physical functioning, vitality, and general health scores among exposed workers and community patients [28,27,25].

Conclusions: Genetic strategies that improve pathway stability, combined with targeted engineering controls at bioaerosol-generating handling steps, address both the yield and occupational-health dimensions of biofuel production inefficiency and represent the most evidence-supported path toward protecting patient quality of life [11,32].

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